薬理と治療

Abstract

Objective We examined the wound−healing effects of an oral mixture containing L−arginineand L−glutamine. The effects of this mixture were compared with that of L−arginine alone onthe daily wound area ratio, sum of daily wound area ratio, and time to healing of decubitusulcers in rats fed a low−protein diet.Methods Decubitus ulcers were experimentally induced in SD rats aged 8 weeks. Animalswere fed a normal protein diet（Labo MR Stock；19％ protein）before the induction of decubitusulcers. They were then fed a low−protein diet（AIN−93 M；12％ protein）ad libitum.Test material AG（mixture containing L−arginine and L−glutamine）, test material A（mixturecontaining L−arginine alone）, or control（distilled water）were given twice a day at a totaldaily dose of L−arginine 500 mg／kg and L−glutamine 200 mg／kg.Results Significant reductions in healing indices, daily wound area ratio, and time to healingoccurred in group AG compared with control group；there are no such differences ingroup AG compared with group A.Conclusions A mixture containing L−arginine and L−glutamine, but not L−arginine alone,accelerated healing in a low−protein breeding decubitus ulcer rat model. When L−arginineand L−glutamine is combined, it shows good effect for the first time.（Jpn Pharmacol Ther 2009；37：745−50）KEY WORDS L−arginine，L−glutamine，Wound healing，Oral administration，Low−proteindiet，Decubitus ulcer, Rats