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薬理と治療
Abstract
The aim of this study was to investigate the effect of ethylenediurea(EDU:N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N-phenylurea)which induced superoxide dismutase(SOD)and catalase(CAT)activities, on linoleic acid hydroperoxide(LHP)-induced PC12 cell injury.PC12 cells were cultured in RPMI 1640 medium with antibiotics. PC12 cell viability wasdetermined by crystal violet(CV)stain 24 hr after 0.05 mM LHP addition. At 6 hr after 0.05mM LHP addition, single-stranded DNA(ssDNA)was determined by ssDNA ApoptosisELISA kit. Caspase-3, SOD-1 and CAT mRNA were determined by Quantitative Real Time-PCR. After LHP addition, PC12 cell viability was significantly decreased to 70% of control(without treatment, p<0.01). The addition of 0.1 mM EDU 1 hr before LHP addition, PC12cell viability returned to 93%. Apoptosis was significantly increased by LHP treatment(447%, p<0.01)and it was significantly decreased to 248% by EDU treatment(p<0.01).Caspase-3 mRNA was increased by LHP treatment(170%), and it was decreased by EDUtreatment(145%). SOD-1 mRNA was increased by LHP, EDU and both treatments:119%,132% and 107% of control, respectively. CAT mRNA was not increased. These results indicatethat EDU exerts a protective effect against LHP-induced PC12 cell injury in partthrough an inhibition of apoptosis.
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