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薬理と治療
- Authors: Masashi Honda1, et al.
Abstract
We studied about the vasorelaxant effect of bonito extract on norepinephrine (NE)induced contract in rat isolated aorta. Furthermore, we attempted to clarify which component plays the major role in the vasodilating effect. Bonito extract concentration-dependently inhibited the contraction induced by NE in thoracic aortae with and without endothelium to a certain equally. In other words, the removal of the endothelium did not affect the relaxing effect of bonito extract upon NE-induced precontraction, suggesting the possibility that this action is related to the inhibitory effect on the constriction of blood vessels induced by NE via alpha adrenergic receptors. To investigate the identity of the active component, we examined the blood vessel-relaxing actions of three different fractions:total bonito extract, components with molecular weights below 1000(Mw<1000 fraction), and chemically-defined components. It was shown that total bonito extract, the Mw<1000 fraction and the mixture of chemically-defined compounds inhibited the contraction induced by NE in the thoracic aortae. The effect of mixture of chemically-defined compounds can contribute 59.2% of the effect of total bonito extract. From these results, it is also a possibility that the active component with molecular weight below 1000 is combination of histidine, carnosine and unknown peptides.
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