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薬理と治療
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Abstract
Objective Cetirigine hydrochloride(CT)and Levocetirizine hydrochloride(LCT)is well known to be non-sedative histamine H1 receptor antagonists and used for the treatment of the allergic diseases, such as pollinosis and atopic dermatitis with remarkable success. However, the influence of these agents on eosinophil degranulation is not fully understood. The present study, therefore, was undertaken to examine the influence of these agents on eosinophil degranulation, which was assessed by measuring eosinophil cationic protein(ECP)and major basic protein(MBP). Methods Mouse peritoneal exudate eosinophils obtained from BALB/c mice infected with Mesocestoides cortii were sensitized with M. cortii-specific mouse IgE. These sensitized eosinophils were stimulated with M. cortii-excreted antigens(1.0 ng/mL)in the presence of various concentrations with either CT or LCT for 24 hours. The levels of ECP and MBP in culture supernatants were examined by ELISA. We also examined the levels of ECP and MBP in nasal secretions obtained from pollinosis patients treated with LCT once a day for 4 weeks at a single dose of 5 mg by ELISA. Results Treatment of eosinophils with CT at more than 0.15 μM could significantly suppress the appearance of ECP and MBP after antigenic stimulation. The addition of LCT into eosinophil cultures at more than 0.05 μM also significantly inhibited the secretion of ECP and MBP from eosinophils induced by antigenic stimulation. Oral administration of LCT caused significant suppression of the appearance of ECP and MBP in nasal secretions along with attenuation of clinical symptoms. Conclusion These results strongly suggest that the suppressive activity of CT and LCT on eosinophil degranulation consists, in part, of a therapeutic mode of action of the agents on allergic diseases such as pollinosis.
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