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薬理と治療
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Abstract
Objectives Eradication of Helicobacter pylori (HP)is performed for treatment in patients with peptic ulcers and chronic gastritis who have upper abdominal symptoms. The confirmation of successful HP eradication can be determined using a urea breath test as a marker of the urease activity of HP.Therefore, administering a drug that inhibits urease activity, like lansoprazole, before the diagnostic test of HP eradication is concerned about the possibility that the diagnosis is false︱negative result, and the prescription for these drugs inhibiting urease activity is limited. In this study, we examined the effect of acotiamide hydrochloride hydrate(acotiamide), acid suppressants, nizatidine, a histamine H2 receptor antagonist, and lansoprazole, a proton pump inhibitor, on the urease activity. Methods Test compound solution, urease solution, and HEPES buffer(pH 7.0)were mixed and incubated for 30 min at 37℃. The mix solution was reacted by incubating at 37℃ after addition of urea solution to the mixture. Fifteen minutes later, the reaction was stopped by adding sulfuric acid. Amount of ammonia induced in the reaction solution was quantified using a modified Fujii︱Okuda method. Results Both acotiamide and nizatidine at 1⊠10-3 mol╱L showed a slightly inhibitory effectof urease activity, but did not inhibit in a concentration of 1⊠10-4 mol╱L or less. Both lansoprazole and benzohydroxamic acid(positive control)inhibited urease activity in a concentration︱dependent manner. The 50% inhibitory concentrations(IC50 values)of lansoprazole and benzohydroxamic acid on urease activity were estimated to be 4.9⊠10-6 mol╱L and 6.0⊠10-6mol╱L, respectively.Conclusions These results indicate that acotiamide as well as nizatidine has no or little effect on urease activity. It is therefore unlikely that acotiamide affects the diagnostic test after HP eradication in clinical setting.
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