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薬理と治療
Abstract
Objective The present study was designed to examine the influence of glucosamine hydrochloride(GH)on the ability of synoviocytes to produce nitric oxide(NO), which is the important effector molecule in the development of osteoarthritis, in response to osteopontin(OPN)stimulation in vitro. Methods Synoviocytes(5⊠105 cells╱mL)derived from osteoarthritis patients were stimulated with 330 ng╱mL OPN in the presence of various concentrations of GH for 24 hours. The levels of NO in culture supernatants was examined by NO2╱ NO3 assay kits. To examine the influence of GH on transcription factor, NF︱κB, activation and iNOS mRNA expression, synoviocytes (5×10 5 cells╱mL)were also cultured in a similar manner for 4 and 12 hours, respectively. The levels of both mRNA expression and transcription factor activation were measured by ELISA. Results Addition of GH into cell cultures caused the suppression of OPN ︱induced NO production from synoviocytes. The minimum concentration that caused significant suppression of NO production was 1.0 mg╱mL. GH at more than 1.0 mg╱mL also inhibited iNOS mRNA expression and NF︱κB activation, which were increased by OPN stimulation in synoviocytes. Conclusion These results strongly suggest that GH favorably modify the clinical condition of osteoarthritis patients through the suppression of NO production from synoviocytes.
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