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薬理と治療
Abstract
Background and aim Jumihaidokuto(JHT)is a traditional Japanese medicine used in the early phases of purulent skin disease and acute dermatoses, and is effective for treatment of urticaria, eczema, and the like. JHT was evaluated in two animal models of ear dermatitis showing acute edema against a stimulus. Methods JHT (0.5, 1, and 2 g╱kg, p. o.)was examined in a model of irritant contact dermatitis(ICD)induced by applying phorbol 12-myristate 13-acetate(PMA), a major constituent of croton oil, to mouse ears. The ear thickness was measured using a dial thickness gauge micrometer 6 h after PMA application. Moreover, in an acute dermatitis model induced by injecting heat-killed Propionibacterium acnes(P. acnes)intradermally into rat ears, the effect of JHT on ear edema was evaluated 2 h after P. acnes injection. Antioxidative activities of JHT, its components and constituents were determined in assays of hydrogen peroxide-dependent oxidation and superoxide generation. Results JHT significantly suppressed cutaneous inflammation induced by PMA or P. acnes, especially showing higher efficacy on dermatitis evoked by P. acnes. JHT showed concentration-dependencies on the two assays of antioxidative action. Of JHTʼs ten components, keigai, bokusoku, and kanzo, exerted antioxidative activity, and six flavonoids contained in the three components exhibited the activity strongly. Conclusions JHT suppressed ICD-like dermatitis and pathogen-induced dermatitis, consistent with the clinical uses of JHT. The flavonoids of keigai, bokusoku, and kanzo exerted antioxidative activities and may contribute to the anti-dermatitis effect of JHT, although it needs further investigation.
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