薬理と治療

Abstract

Background and aim The Mediterranean diet is reported to be effective in preventing cardiovascular disease（CVD）. Olive polyphenols, grape seed oligomeric proanthocyanidins and vitamin C are major components of the Mediterranean diet. We evaluated combination effects of these compounds on vascular endothelial function as a surrogate marker for arteriosclerosis and CVD. Methods A randomized, placebo-controlled, double-blind crossover trial was conducted. Seventy-two healthy subjects were randomly assigned to the test food group（50 mg of olive extract containing 6 mg of hydroxytyrosol, 100 mg of grape seed extract containing 60 mg of oligomeric proanthocyanidins and 100 mg of vitamin C）or the placebo group. The subject ingested each supplement for 4 weeks. After a washout period of 2 weeks, the other supplement was ingested for 4 weeks. Results Flow-mediated vasodilation（FMD）was significantly improved by 4 weeks supplementationof the test food, and the change in FMD from baseline to 4 weeks was also significantly greater in the test food group than in the placebo group. Urinary 8-OHdG, an oxidative stress marker, and plasma high-sensitivity C-reactive protein（hsCRP）, an inflammatory marker, were significantly reduced in the test food group compared to the placebo group. No adverse events relating to test food supplementation were observed. Conclusions These results suggest that the supplementation of olive and grape seed polyphenols with vitamin C could improve endothelial function via anti-oxidant and anti-inflamma-tory functions and that might be safe and effective for reducing the risk of arteriosclerosis and CVD.