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In vitro における人参養栄湯のミトコンドリア量に対する作用
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JPY
Abstract
Background This study was carried out to elucidate effects of ninjinyoeito, Japanese herbal medicine, on the mitochondria amount as one of clarifying the mechanism of action on the anti-fatigue effect. Methods Effects of ninjinyoeito on cell counts and mitochondria contents by using MTS assay or Mitotracker green in cultured HUVEC were determined in the in vitro assay. Results Ninjinyoeito enhanced formazan synthesis under the conditions with both growth phase and confluent cultured HUVEC with no influence on cell proliferation. In addition, ninjinyoeito increaed mitochondria content. Conclusion It is likely appeared that effecancy of ninjinyoeito may be mediated by the increased mitochondria contents in HUVEC.
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