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薬理と治療
Abstract
Objective Recovery effect of a peptidoheteroglycan prepared from Chlorella pyrenoidosa on the number of blood cells and hematopoietic progenitor cells in the irradiated mice were investigated. Methods (1)Serum biochemical investigation in non︱irradiated group, 5.0 Gy irradiated group or 5.0 Gy irradiation plus peptidoheteroglycan(10 mg╱kg⊠2╱day)treated group were examined. (2)The number of spleen and╱or bone marrow cells in the dependent of the level of erythroid burst-forming cell(BFU-E), colony-forming unit-erythropoetin(CFU-E)dependent, and granulocytes- macrophage colony-forming cell(CFU-GM). (3)An examination on hematological test and hemopoietic progenitor calls were perfomed in 10 cases of each group. (4)For the histopathological examination, the organs of spleen and thymus were fixed 10% formalin, embedded in paraffin and cut in slices(6μm thick), then stained with hematoxylin-eosin. Microscopic examinations were performed on 3 samples each of the groups at 11 days after administration. Results 5.0 Gy x-irradiated mice induced the decrease of platelet, white blood cell, granulocytes, lymphocytes and monocytes. Intraperitoneally administration of peptidoheteroglycan (PHG)tended to decrease these damage on hematopoiesis and PHG was increased significantly on the number of spleen and bone marrow cells in the dependent of BFU-E and CFU-E. A markedly elevation in DFU-GM level was observed. Histological examinations revealed that PHG accerelated the hematopoietic recovery and decrease on damage of spleen and thymus. Conclusions These results at least that PHG might be one of the valuable material in preventive effect on the numbers of blood cells and hematopoietic progenitor cells suppression by radiation injury.
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