薬理と治療

Abstract

Objective In this study, we investigated the impact of polyphenols on vascular endothelial and renal functions associated with the development of swelling. We studied the effects of the polyphenol αG hesperidin PA-LE on cellular function in human umbilical vein endothelial cells （HUVEC）and human renal proximal tubular epithelial cells（HRPTEpC）. Methods αG hesperidin PA-LE was added to HUVEC and HRPTEpC cultures at final concentrations of 1.25％, 0.625％, 0.312％, and 0.156％. To assess vascular endothelial function, we measured the expression of nitric oxide（NO）synthase and NO production in HUVEC. To evaluate renal function, we measured Na＋╱K＋ATPase activity and expression level using HRPTEpC. Results Three and seven days treatment with αG hesperidin PA-LE increased endothelial NO synthase（eNOS）gene expression and NO production in HUVEC and suppressed gene expression of Na＋╱K＋ATPase and inhibited Na ＋╱K＋ATPase protein accumulation in HRPTEpC. Conclusions These results suggest that αG hesperidin PA-LE increases NO production via the expression of the eNOS gene in HUVEC and suppresses Na＋╱K＋ATPase gene expression in HRPTEpC, thus inhibiting the accumulation of Na＋╱K＋ATPase proteins.