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薬理と治療
Abstract
Objective Supplementation with 5-aminolevulinic acid(ALA)has previously demonstrated an increase in dermal collagen density and skin hydration in middle-aged women. This study aimed to provide further insight into this observation by investigating the effects of ALA on collagen and hyaluronic acid production in cultured normal human dermal fibroblasts (NHDFs). Methods NHDFs were treated with various concentrations of ALA for 48-72 h to determine cell growth, collagen and hyaluronic acid production, and mitochondrial number and activity. Results ALA promoted collagen and hyaluronic acid production at 2-20μM, which did not affect NHDF growth. Although ALA increased the mitochondrial number and activity, the concentration of ALA required for these effects were relatively higher(>200μM). Therefore, the effects of ALA on collagen and hyaluronic acid production may be distinct from those on mitochondrial number and activity. Conclusion The previous observation that supplementation with ALA increases dermal collagen density and skin hydration may be partly explained by the stimulation of collagen and hyaluronic acid production in dermal fibroblasts.
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