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薬理と治療
Abstract
Background In previous studies, we confirmed that administration of Amazake to healthy adult women for 5 weeks, improved sagging pores and sebum quantity in skin. Objectives In this study, we investigated the effects of hot water extracts of Sake Cake(SH)and Rice Koji(KH), respectively, on fibroblast-mediated collagen gel contraction. We further examined their effects, on regulation of matrix metalloproteinases(MMP)and lipid production, and on improving sagging pores, in cultured sebocytes. Methods Effects of SH, KH, or a 1:2 Sake Cake extract: Rice Koji extract(SK mix), were evaluated. Collagen gel contraction by fibroblasts was assessed. Production of ProMMP-2 and ProMMP-9 in sebocytes was examined by gelatin-zymography. Triglyceride and fatty acid production in sebocytes was examined using thin-layer chromatography. Results The contractility of collagen gels significantly increased upon treating fibroblasts with SH, KH, or SK mix. KH and SK mix significantly inhibited ProMMP-2 production. Triglyceride production was reduced in sebocytes treated with SH(P<0.05)and SK mix(P=0.070). Conclusions Our study suggests that Sake Cake and Rice Koji improve sagging pores and control sebum quantity by activating collagen-binding to extracellular matrix and inhibiting MMP and triglyceride production.
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