薬理と治療

Abstract

Objectives　The present study aimed to validate the antioxidant effect of Liquid of Fermented Plant Extract SW（LFPE-SW）, containing numerous fruits and vegetables. Methods　We evaluated the antioxidant capacity of LFPE-SW using superoxide radicals, hydroxyl radicals, hydrogen peroxide, and free radicals. The scavenging ability of superoxide radicals was evaluated as the superoxide dismutase（SOD）-like activity. We calculated the impact on hydroxyl radicals from the hydroxyl radical antioxidant capacity（HORAC）value. Furthermore, we assessed the eliminating ability of hydrogen peroxides from their catalase-like activity and measured the scavenging ability of free radicals using 2,2-diphenyl-1-picrylhydrazyl（DPPH）. Results　LFPE-SW scavenged superoxide radicals at ≤0.5％ concentration. In addition, LFPE-SW scavenged hydroxyl radicals in a dose-dependent manner, and the HORAC value was 161.9μM GAE╱g. While LFPE -SW at ≥2％ concentration eliminated hydrogen peroxides, ≥1％ exhibited the scavenging activity for DPPH radicals. Conclusions　Although LFPE-SW exhibits low antioxidant ability for hydrogen peroxides, it displays high antioxidant ability in superoxide radicals, hydroxyl radicals, and DPPH radicals, suggesting that LFPE-SW exerts antioxidant effects.