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ヒト腸上皮細胞における植物発酵ペーストSW の抗炎症効果
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JPY
Abstract
Objective The aim of this study was to examine the anti-inflammatory effect of Paste of Fermented Plant Extract SW(PFPE-SW)on human intestinal epithelial cells. Methods The intestinal epithelial cell line, Caco-2, was differentiated into intestinal epithelial-like cells. PFPE-SW was added to MEM medium(with Earleʼs Salts and L-Glutamine)at a final concentration of 0.5%, 1%, and 2% for 24 hours at 37℃ in 5% CO2. Then, this medium was replaced with a medium containing PFPE-SW in the presence or absence of 100 ng╱mL TNF-α for 24 hours at 37℃ in 5% CO2. The supernatant was collected, and the concentration of interleukin-8(IL-8)was determined using the enzyme-linked immunosorbent assay. Results PFPE-SW inhibited TNF-α-mediated IL-8 production in a concentration-dependent manner(P<0.05). Even without TNF-α stimulation, at concentrations of 2% and 0.5%, PFPE-SW significantly suppressed IL-8 production(P<0.05). Conclusions PFPE-SW suppressed TNF-α-dependent and TNF-α-independent IL-8 production indicating the anti-inflammatory potential of PFPE-SW on human intestinal epithelial cells.
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