薬理と治療

Abstract

Objective　The aim of this study was to examine the anti-inflammatory effect of Paste of Fermented Plant Extract SW（PFPE-SW）on human intestinal epithelial cells. Methods　The intestinal epithelial cell line, Caco-2, was differentiated into intestinal epithelial-like cells. PFPE-SW was added to MEM medium（with Earleʼs Salts and L-Glutamine）at a final concentration of 0.5％, 1％, and 2％ for 24 hours at 37℃ in 5％ CO2. Then, this medium was replaced with a medium containing PFPE-SW in the presence or absence of 100 ng╱mL TNF-α for 24 hours at 37℃ in 5％ CO2. The supernatant was collected, and the concentration of interleukin-8（IL-8）was determined using the enzyme-linked immunosorbent assay. Results　PFPE-SW inhibited TNF-α-mediated IL-8 production in a concentration-dependent manner（P＜0.05）. Even without TNF-α stimulation, at concentrations of 2％ and 0.5％, PFPE-SW significantly suppressed IL-8 production（P＜0.05）. Conclusions　PFPE-SW suppressed TNF-α-dependent and TNF-α-independent IL-8 production indicating the anti-inflammatory potential of PFPE-SW on human intestinal epithelial cells.