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薬理と治療
Abstract
Objective The influence of fragrance components on the development of allergic rhinitis induced by toluene 2, 4-diisocyanate(TDI)were examined in F344 rats.Methods F344 male rats(6-weeks of age)were sensitized by intranasal instillation of 10% TDI once a day for 5 days. These rats were exposed to various concentrations of fragrance components, α-n-hexyl cinnamic aldehyde(αHCA), 2-phenethyl ITC(2-PITC), n-butyl ITC (nITC), benzyl ITC(bITC), amyl salicylate(AS), methyl jasmonate(MJ), citral, muscone for 4 hours/day for 5 consecutive days, which was started 10 days after final TDI instillation. Nasal symptoms, sneezes and nasal rubs for 10 minutes, induced by 10% TDI nasal challenge were evaluated 24 hours after fragrance component exposures. To examine the influence of fragrance components on the appearance of neuropeptide, substance P(SP), calcitonin gene-related peptide(CGRP)and nerve growth factor(NGF)in nasal mucosa, nasal lavage fluids were obtained from rats 60 min after 10% TDI nasal challenge, and the levels of neuropeptides were examined by ELISA. Results HCA, PITC, nITC and bITC, but not AS, MJ, citral and muscone at 0.1% could attenuate the appearance of nasal symptoms and decreased the number of both sneezes and nasal rubs. We then examined the concentrations of neuropeptides, such as SP, CGRP, NGF in nasal lavage fluids obtained from TDI-sensitized and fragrance components-inhaled rats 60 minutes after TDI challenge. Exposure of TDI-sensitized rats to HCA, PITC, nITC and bITC could suppress the increase in SP, CGRP and NGF levels in nasal lavage fluids, which were increased by TDI challenge. On the other hand, AS, MJ, citral and muscone scarcely affected the appearance of neuropeptides in nasal lavage fluids. Conclusion These results clearly showed that exposure to HCA, PITC, nITC and bITC, which are delivatives of cinnamic aldehyde or allyl isothiocyanate could attenuate the develop ment of allergic nasal symptoms through the suppression of neuropeptide productions in nasal walls.
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