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薬理と治療
Abstract
Objectives The presented study aimed to investigate whether spermatozoa treated with water-soluble yeast-fermented garlic extract, a novel processed garlic food, affect fertilization and early embryogenesis. Methods Spermatozoa and oocytes were collected from BALB/cAJcl male mice(12-week-old)and superovulated C57BL/6 J female mice(4-week-old), respectively. Spermatozoa were pre-treated with water-soluble extraction of yeast fermented garlic, and then used for in vitro fertilization(IVF). On the following day, the rates of fertilized 2-cell stage embryos, unfertilized oocytes and dead oocytes to total oocytes used for IVF were calculated. After a further 3 days, rates of blastocysts, 2-cell stage embryos and dead embryos to 2-cell stage embryos were calculated. Results Spermatozoa treated with yeast-fermented garlic extract did not affect fertilization potential such as the number of fertilized 2-cell stage embryos, unfertilized eggs, and dead eggs, but significantly decreased the percentages of 2-cell block during mouse early embryogenesis, leading to significant increase of early embryos that developed up to the blastocyst stage. Conclusions Yeast-fermented garlic food, a novel processed food, promotes early embryogenesis beyond 2-cell block of mouse embryos, which may be potential seeds for improving IVF in infertility treatment.
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