薬理と治療

Abstract

Objective　In this study, we investigated the inhibitory effect of Liquid of Fermented Plant Extract SW contained low molecular collagen（hereinafter referred to as LFPE-SW）, a mixture of various fruits and vegetables, on melanogenesis using B16 melanoma cells. Methods　The antioxidant capacity of LFPE-SW was evaluated based on its scavenging ability against superoxide radicals, hydrogen peroxide and free radicals. The inhibition of melanin production was assessed from the amount of melanin produced using B16 melanoma cells. Furthermore, the rate of inhibition of tyrosinase activity and tyrosinase gene expression levels were evaluated. Results　LFPE-SW treatment significantly reduced melanin production in melanocytes. Furthermore, LFPE-SW showed scavenging ability against superoxide radicals, hydrogen peroxide and free radicals. LFPE-SW showed a significant tyrosinase inhibitory effect in vitro, while the expression of tyrosinase mRNA in the B16 melanoma cells did not change after LFPE-SW treatment. Conclusions　These findings suggest that the inhibitory effect of LFPE-SW on melanogenesis is due to the inhibition of tyrosinase activity.