Full text loading...
Current Herpetology
- Previous Article
- Table of Contents
- Next Article
Abstract
Abstract:
We developed 18 novel microsatellite markers for Onychodactylus japonicus using Illumina Miseq System, and tried cross-amplifications in three congeners. The number of alleles and expected heterozygosity at each locus in O. japonicus were 3–15 (mean=6.83) and 0.45–0.89 (mean=0.73), respectively. Cross-amplifications in congeners succeeded in 9–13 of 18 loci, and eight loci were consistently amplified across all four species. The markers described here will be useful not only for investigating phylogeography and fine-scale genetic structure, but also for estimating genetic diversity of endangered species or populations for conservation management.
We thank I. Hirano, O. Inaba, and K. Watanabe for help in collecting specimens. We are also grateful to two anonymous reviewers for valuable comments on the earlier version of manuscript. All DNA samples used in this study are stored in the Center for Molecular Biodiversity Research, National Museum of Nature and Science, Tokyo. This work was supported by JSPS KAKENHI Grant Number JP26870320 and the Capital of Mother Nature Tadami Academic research project to NY.
Data & Media loading...