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Current Herpetology
Abstract
Abstract:
We isolated and characterized 11 microsatellite markers for Ovophis okinavensis, a viperid species endemic to the Ryukyu Archipelago. These markers showed polymorphism among 25 individuals from Okinawajima Island. The number of alleles per locus was 3–11, and observed and expected heterozygosity were 0.240–0.960 and 0.218–0.853, respectively. Cross-amplification confirmed that at least seven out of the 11 microsatellite markers were applicable to a putative relative, Trimeresurus gracilis. The markers provided here are expected to be valuable for population- and/or individual-level genetic studies of not only O. okinavenesis, but also its relatives.
We would like to thank M.-C. Tu and C.-F. Lin for collecting and providing valuable tissue samples of T. gracilis. The samples were from protected animals in Taiwan. They were permitted to collect by Dr. Tu who obtained his permission from Council of Agriculture, Executive Yuan; and T. Hikida and T. Okamoto for their technical support. We also thank anonymous reviewers for their comments in improving our manuscript. This research was financially supported in part by the Global COE Program A06 to Kyoto University.
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