薬理と治療

Abstract

Globin digest（GD）that produced from enzymatic hydrolysis of globin protein in hemoglobin suppressed postprandial elevations of chylomicron triglycerides in man. High lipoprotein remnant level after fat ingestion is a risk factor for atherosclerosis. Effect of GD was studied on postprandial remnant−like particles（RLP）as an index of lipoprotein remnant and on lipoprotein lipase（LPL）and hepatic triglyceride lipase（HTGL）activities. Twelve healthy subjects attended to a placebo−controlled crossover study. The subjects ingested a control high fat diet with or without GD. The increases of RLP concentration after ingestion of GD 1 g were significantly lower than the control. The areas under the curves of RLP during 6h of GD group were 30−50％ lower than the corresponding control group. Another placebo−controlled crossover trial was performed using the same diet to examine the influence of GD on LPL and HTGL activities in the healthy participants. The LPL and HTGL in the subjects were activated by GD supplementation. Activation of the lipases by GD was indirect action through inner activators. When the subjects ingested GD 3 g, serum insulin levels temporary increased than the control. The activation of lipases seemed to occur through insulin’s action. Pancreatic lipase activities were reduced dose−dependently by GD in vitro. Suppression of pancreatic lipase and activation of LPL and HTGL by GD might be contributed to the reduction of postprandial RLP levels. GD seems to be useful for lowering incidence of cardiovascular diseases and atherosclerosis.（Jpn Pharmacol Ther 2008；36：531−40）KEY WORDS Globin digest, Remnant−like particles, Lipoprotein lipase, Hepatic triglyceride lipase, Insulin