薬理と治療

Abstract

The aim of this study was to investigate the effect of ethylenediurea（EDU：N－［2－（2－oxo－1－imidazolidinyl）ethyl］－N－phenylurea）which induced superoxide dismutase（SOD）and catalase（CAT）activities, on linoleic acid hydroperoxide（LHP）－induced PC12 cell injury.PC12 cells were cultured in RPMI 1640 medium with antibiotics. PC12 cell viability wasdetermined by crystal violet（CV）stain 24 hr after 0.05 mM LHP addition. At 6 hr after 0.05mM LHP addition, single－stranded DNA（ssDNA）was determined by ssDNA ApoptosisELISA kit. Caspase－3, SOD－1 and CAT mRNA were determined by Quantitative Real Time－PCR. After LHP addition, PC12 cell viability was significantly decreased to 70％ of control（without treatment, p＜0.01）. The addition of 0.1 mM EDU 1 hr before LHP addition, PC12cell viability returned to 93％. Apoptosis was significantly increased by LHP treatment（447％, p＜0.01）and it was significantly decreased to 248％ by EDU treatment（p＜0.01）.Caspase－3 mRNA was increased by LHP treatment（170％）, and it was decreased by EDUtreatment（145％）. SOD－1 mRNA was increased by LHP, EDU and both treatments：119％,132％ and 107％ of control, respectively. CAT mRNA was not increased. These results indicatethat EDU exerts a protective effect against LHP－induced PC12 cell injury in partthrough an inhibition of apoptosis.