薬理と治療

Abstract

Objective We examined effect of placenta extract on the proliferation and TypeⅠcollagen mRNA expression of human skin fibroblast. Methods Normal human skin fibroblast were treated with 0.16, 0.32, 0.64％ placenta extract. After 48 hours, the proliferation of skin fibroblast was determined by Cell Counting Kit︱8. For the expression of COL1A1, which encodes the major component of type Ⅰ collagen, total RNA was isolated from skin fibroblast treated with placenta extract for 48 hours and analyzed using RT︱qPCR. Results Placenta extract stimulated the proliferation of human skin fibroblast in a dose︱dependent manner. Further, the expression of COL1A1 gene was also promoted by placenta extract. Conclusion These results indicated that placenta extract has effective components on the activation of skin fibroblast, and this activation of fibroblast may be contribute to recovery of wrinkle and wound healing.