薬理と治療

Abstract

Objective We wanted to verify clinically the relationship between absorption of anthocyanins （those plasma concentrations）and eye fatigue by using Standardized Bilberry Extract（SBE）. Methods We performed a placebo-controlled, double-blind, randomized, and crossover clinical trial. Subjects were healthy males and females from 20 to 44 years old. Either 180 mg of SBE or placebo per day was administered orally for 7 days. The wash-out period for the crossover trial was 13days. At the end of the 7-day trial, subjects wore an eye mask for 10 minutes before playing a video game（tetris） an a smartphone for 30 minutes to induce VDT stress. It was evaluated HFC-1 values as an objective index, and questionnaire as a subjective index. The day after the consecutive 7-day trial, the subjects plasma concentration of anthocyanins was measured 1 hr after a administration of SBE or placebo. Result HFC-1 values of the subjects administered SBE showed significant improvement （P＝0.012）compared with those administered placebo. Moreover, plasma concentration of anthocyanins were correlated with HFC-1 values（r＝－0.369）, and subjective index by questionnaire（r＝－0.530）. Conclusion Administration of SBE for 7 days increase plasma concentration of anthocyanins, and improved the eye fatigue induced by VDT stress and╱or nearsighted work.