薬理と治療

Abstract

Objective The present study was designed to examine the influence of hyaluronic acid（HA） on the ability of synoviocytes to produce periostin, which is one of important effector molecule in the development of osteoarthritis, in response to IL︱13 stimulation in vitro. Methods Synoviocytes（1×105cells╱mL）derived from an osteoarthritis patient were stimulated with 10.0 ng╱mL IL-13 in the presence of various concentrations of HA for 48 hours. The levels of periostin in culture supernatants was examined by ELISA. To examine the influence of HA on transcription factor, STAT6, activation and periostin mRNA expression, synoviocytes （1×105cells╱mL）were also cultured in a similar manner for 12 and　 24 hours, respectively.　STAT6 activation was examined by ELISA and the levels of mRNA expression was real-time　RT-PCR. Results Addition of HA into cell cultures caused the suppression of IL-13-induced periostin production from synoviocytes. The minimum concentration that caused significant suppression of periostin production was 5.0 mg╱mL. HA at more than 5.0 mg╱mL also inhibited STAT6 activation and periostin mRNA expression, which were increased by periostin stimulation in synoviocytes. Conclusion These results strongly suggest that HA favorably modify the clinical condition of osteoarthritis patients through the suppression of periostin production from synoviocytes after IL-13 stimulation.