薬理と治療

Abstract

Background Our earlier reports have indicated that pre-exercise ingestion of an amino acid mixture composed of arginine, alanine, and phenylalanine（A-mix） increases levels of blood ketone bodies during exercise by increasing secretion of glucagon in overweight humans. Although these results suggest A-mix supplementation enhances lipolysis during exercise, acute stimulation of glucagon secretion may impair the health of diabetic patients. They cannot regulate glucagon secretion due to insulin resistance and╱or insulin deficiency, which might lead to the abnormally elevated blood levels of glucose and hemoglobinA1c（HbA1c）. Herein, we carry out the long-term effect of A-mix with exercise in a rat model of type 2 diabetes and obesity on blood glucose level. Methods Male Zucker diabetic fatty（ZDF）rats underwent swimming exercise（3 days╱week） with（n＝5）or without（n＝5）A-mix supplementation（1 g╱kg body weight╱day）over a 5-week period. Results There were no difference in body weight, food intake, blood levels of glucose, HbA1c, insulin, glucagon, weights of liver, soleus, plantaris, gastrocnemius, epididymal and mesenteric fat. Although A-mix group exhibited significantly higher blood level of ketone bodies compared to control group at week 1, there were no difference at week 2 and later between two groups. Conclusions These results suggest that continuous supplementation of A︱mix（1 g╱kg body weight╱day）combined with exercise does not elevate the levels of casual blood glucose nor HbA1c in a rat model of type 2 diabetes and obesity.