薬理と治療

Abstract

Objective　To investigate the anti-inflammatory effects of Liquid of Fermented Plant Extract SW, including low-molecular-weight collagen（LFPE-SW）, containing various fruits and vegetables. Methods　Differentiated macrophage precursor cells （THP-1 cell line）were stimulated using lipopolysaccharide（LPS）, which caused a pseudo-immune reaction to promote the secretion of proinflammatory cytokines and growth factors. LFPE-SW was added to RPMI-1640 medium at final concentrations of 1％ and 5％ in the presence of 1μg╱mL LPS. After 2, 5, and 24 h of treatment, the anti-inflammatory effects were determined by measuring the expression levels of genes encoding proinflammatory cytokines（TNF-α, IL-1, and IL-6）and growth factors（FGF-1, FGF-2, and EGF）using real-time RT-PCR. Results　TNF-α expression levels were significantly decreased after short-term and long-term LPS stimulations for 2 and 5 h, respectively. Additionally, IL-1 expression levels were significantly decreased after short-（2 h）, medium-（5 h）, and long-term（24 h） stimulations. Furthermore, IL-6 expression levels were significantly suppressed by long-term stimulation. No remarkable changes were identified in FGF-1 and EGF expression levels, whereas FGF-2 expression levels were significantly increased after medium-and long-term stimulations. Conclusions　LFPE-SW suppresses the expression of various proinflammatory cytokines under long-term stimulation. Moreover, it increases the expression of FGF-2 and fibroblast growth factor, and therefore, it may promote tissue repair.