薬理と治療

Abstract

Objective　The aim of this study was to investigate the effect of a dietary supplement containing porcine skin collagen peptides on hair on healthy subjects. Moreover, to discuss the mechanism on these effects, we evaluated the effects of treatment with collagen peptides in human follicle dermal papilla cells. Methods 　①A randomized, double-blind, placebo-controlled parallel-group, comparison study was conducted in 65 healthy subjects（20︱69 years old）. The subjects were randomized into two groups and given either the test product（125 mL drink containing 10 g collagen peptides derived from porcine skin）per day or the control product（placebo drink）for 8 weeks. Visual analogue scale（VAS）for hair and two dimension mood scale（TDMS-ST）were evaluated. ②We also evaluated whether hydroxyproline（Hyp）, prolyl-hydroxyproline（Pro-Hyp）, and glycyl-prolyl-hydroxyproline（Gly-Pro-Hyp）promote the proliferation of dermal papilla cells and induce the mRNA expression level of vascular endothelial growth factor （VEGF）and fibroblast growth factor-7（FGF-7）in human follicle dermal papilla cells. Results　①The VAS score onʻless hair lossʼandʻstrong hairʼ, and the TDMS-ST score on ʻlivelyʼandʻvitalityʼwere significantly improved at 8 weeks in the test product group who took collagen peptides compared to the placebo group. ②Hyp and Pro-Hyp enhanced cell proliferation, and Pro-Hyp and Gly-Pro-Hyp upregulated the mRNA expression of VEGF in human follicle dermal papilla cells. Conclusions　The results suggested that daily supplementation of collagen peptides derived from porcine skin may be effective for hair by stimulating cell proliferation and the upregulation of VEGF expression（. UMIN-CTR000036892）