薬理と治療

Abstract

Objectives　The aim of the presented study was to examine the effect of Liquid of Fermented Plant Extract SW（LFPE-SW）on human fibroblasts to cellular oxidative stress and collagen gene expression. Methods　Immortalized human fibroblasts （OUMS-36T-1 cells）were cultured at 37℃ in a 5％ CO2 atmosphere in DMEM containing 10％ FBS for 24 h, and then LFPE-SW was added at the concentration ranges of 0.01-10％ for 24 h. The cell survival rate was measured by Cell Counting Kit-8. The cellular reactive oxygen species（ROS）levels were analyzed with fluorescence microscope using CM-H2DCFDA. The gene expression levels of COL1A1 and COL3A1 were quantified using real-time RT-PCR. Results　LFPE-SW showed no cytotoxicity in OUMS-36T-1 cells at the concentration ranges of 0.01-1％ for 24 h, and it significantly decreased cellular ROS levels under normal culture condition. LFPE-SW also prevented H2O2-induced oxidative stress in OUMS-36T-1 cells. In addition, the expression of COL3A1, but not COL1A1, was significantly increased in OUMS-36 T-1 cells treated with LFPE-SW for 24 h. Conclusions　LFPE-SW has the suppressive effect of ROS levels, which might protect human fibroblast cells from oxidative stress. LFPE-SW also increases COL3A1 expression, suggesting that it may affect collagen biosynthesis.