薬理と治療

Abstract

Objective　We examined the antioxidant effects of hot water extract from Chlorella pyrenoidosa（CPE）on free radicals. Methods　The antioxidant in vitro effect was determined by using Electron Spin Resonance （ESR）spectrometory method with a spin trapping agent DMPO（5,5-dimethyl-1-pyrroline-1-oxide）. Results　CPE scavenged dose-dependently ・OH, O2 － and DPPH radicals. We found that CPE had scavenging activity on human oxidative low density lipoprotein. The in vivo effect of CPE on blood plasma antioxidant status of tumor-bearing mice were investigated. Lipid peroxide level（as thiobarbituric acid reactive substances）decreased significantly in the plasma from 3-methylcholanthrene（MC）induced tumor-bearing mice or pulmonary tumor 7423（P・7423）-bearing mice. The metastasis of P・7423 and the ratio of carcinogenesis of MC induced tumor were inhibited. These findings suggest that oral administration of CPE can induce antioxidant effect in the mice blood plasma. Conclusions　These results suggest that CPE might reduce the risk in many human disease such as cancer, atherosclerosis, etc.