薬理と治療

Abstract

Background　Periostin is expressed in various kinds of cancer cells or surrounding tissue and is a factor that accelerates cancer metastasis. Our previous work reported that the herbal medicine juzentaihoto suppressed B16-F10 mouse melanoma cell lung metastasis. The study aims to examine the ability of mouse melanoma cells to secrete periostin, to ascertain the role of secreted periostin, and to determine whether periostin affects the suppression of cancer metastasis on juzentaihoto. Materials and Methods　C57BL╱6 male mice were intraperitoneally injected with B16-F10 mouse melanoma cells and given chow containing 1％ juzentaihoto. In subsequent in vivo experiments, we assessed tumor colony formation in the lung. Additionally, we determined if juzentaihoto suppressed cell migration. Results　Juzentaihoto significantly suppressed B16-F10 mouse melanoma cell metastasis and secretion of periostin in vivo and in vitro and B16-F10 mouse melanoma migration. Conclusions　These results suggested that periostin promoted B16-F10 mouse melanoma metastasis and showed that juzentaihoto suppressed periostin secretion and B16-F10 mouse melanoma metastasis. （Jpn Pharmacol Ther 2022；50：983‒91）