薬理と治療

Abstract

Objective　The efficacy of postprandial serum TG responses of the insoluble fraction from pulverized sesame seeds（LipiSmart®）was evaluated in rats. Furthermore, the mechanism of action of the insoluble fraction in the digestive tract was also investigated in vitro. Methods　After acclimation, the rats were randomly divided into two groups and fasted overnight prior to the High fat diet（HFD）challenge. The insoluble fraction was administrated at a dose of 45 mg/kg body weight using gastrostomy tubes 10 min prior to the administration of the HFD（0h）. Blood samples were collected from the tail vein at 0h, 1.5h, 3h, 4.5h, 6h and 7.5h. The bile acids and oleic acid and monoolein were mixed and homogenized for micellization. The purified insoluble fraction was added to the suspension of bile acid micelles and the supernatant（sup）was collected and lyophilized. After adsorption, oleic acids and monoolein in the sup were measured by LC–MS/MS. Results　Three hours after oral administration of HFD with/without insoluble fraction, the rats that received the insoluble fraction exhibited significantly reduced serum TG concentration compared to the vehicle group. After 4.5 hours, the postprandial serum TG concentration in the insoluble fraction group was still lower than that of the vehicle group. The insoluble fraction demonstrated significant adsorption ability for both micellized oleic acid and monoolein. Conclusion　The purified insoluble fraction was effective in suppressing the postprandial elevation of TG levels in rats following an oral fat load. Furthermore, insoluble fraction indicated high adsorption ability of oleic acid and Monoolein in vitro. （Jpn Pharmacol Ther 2023；51：203‒8）